Detection of tick-borne pathogens by molecular methods
نویسندگان
چکیده
منابع مشابه
Detection of tick-borne pathogens by MassTag polymerase chain reaction.
MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and impl...
متن کاملMolecular detection and treatment of tick-borne pathogens in domestic dogs in Khon Kaen, northeastern Thailand.
We determined the prevalence of tick-borne pathogens in domestic dogs using microscopy and polymerase chain reaction (PCR) techniques. A total of 303 EDTA blood samples were collected from domestic dogs in Khon Kaen Province, Thailand, in May 2013. Microscopic observation of Giemsa-stained smears and molecular diagnosis using conventional PCR were performed. Infected dogs were treated with imid...
متن کاملMolecular Detection and Characterization of Tick-borne Pathogens in Dogs and Ticks from Nigeria
BACKGROUND Only limited information is currently available on the prevalence of vector borne and zoonotic pathogens in dogs and ticks in Nigeria. The aim of this study was to use molecular techniques to detect and characterize vector borne pathogens in dogs and ticks from Nigeria. METHODOLOGY/PRINCIPAL FINDINGS Blood samples and ticks (Rhipicephalus sanguineus, Rhipicephalus turanicus and Hea...
متن کاملMolecular detection of tick-borne pathogens in cattle from Southwestern Ethiopia
Tick-borne diseases (TBDs) cause significant losses among livestock and impact the livelihoods of resource-poor farming communities worldwide. In Ethiopia, detailed studies on the epidemiology of tick-borne pathogens (TBPs) in cattle using sensitive molecular detection methods are scarce. The objective of this study was to determine the prevalence and species composition of bovine TBPs of veter...
متن کاملFirst molecular detection of tick-borne pathogens in dogs from Jiangxi, China
In this study, blood samples obtained from 162 dogs in Jiangxi, China, were employed in molecular screening of canine tick-borne pathogens by PCR and sequencing. Babesia spp. gene fragment was detected in 12 (7.41%) dogs. All samples were negative for Hepatozoon spp., Ehrlichia canis, Coxiella spp., Borrelia spp., Rickettsia spp. and Anaplasma platys. Species-specific PCR analysis further confi...
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ژورنال
عنوان ژورنال: Biologija
سال: 2008
ISSN: 1392-0146,2029-0578
DOI: 10.2478/v10054-008-0040-6